mouse ril-17a Search Results


mouse ril17a  (Abnova)
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Abnova mouse ril17a
IL17A affects fibrotic reactions in pancreatic cancer and differently shapes stroma. (A) Tumor pancreatic fibrosis quantification in KPC/IL17A+/+ (white floating bars) and KPC/IL17A−/− mice (gray floating bars). Each symbol corresponds to a single mouse analyzed with ImageJ (n = 5 to 8 mice per group), and minimum to maximum values are represented. *P < 0.05, **P < 0.001, and ***P < 0.0001 represent significant differences in the IL17A-deficient mice compared with the IL17A-proficient mice. (B) Representative pictures of Picrosirius red staining (Upper) and of SHG microscopy analysis (Lower) of pancreatic tissues from KPC/IL17A+/+ and KPC/IL17A−/− mice at the indicated age (n = 5 to 8 mice per group). The same areas were recorded in transmission light for Picrosirius red staining and with two-photon microscopy for SHG, and collagen fibers are represented in yellow. Arrows indicate more dispersed and less compact collagen fibers, and arrowheads indicate straight and tight collagen fibers. (Scale bars, 75 μm.) (C) Representative pictures of Picrosirius red staining of pancreatic tissues from patients with high (Left) and low (Right) levels of serum IL17A. Magnification at 40× points out the differences in fibrillar structure. (Scale bars, 150 and 50 μm, respectively, at 10 and 40× magnification.) (D) A close-up view of specific genes and proteins differentially expressed in the absence of IL17A. The most relevant factors related to myeloid/granulocyte recruitment and activation (Upper Left), Th1 recruitment and activation (Lower Left), matrix remodeling (Upper Right), and cell growth (Lower Right) are grouped into four blocks. The color-coded maps represent the fold change in IL17A−/− CAFs versus IL17A+/+ CAFs and in IL17A−/− CAFs stimulated with <t>rIL17A</t> (10 ng/mL) versus IL17A−/− CAFs, as indicated by each closed block (n = 3 CAF bulks each). (E) Top upstream regulators predicted by IPA to be activated in the absence of IL17A. (F) Kaplan–Meier curves comparing survival of PDA patients from TCGA interrogation about genes found up-regulated (Th1 response) or down-modulated (myeloid/granulocyte recruitment) in fibroblasts from KPC/IL17A−/− mice.
Mouse Ril17a, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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IL17A affects fibrotic reactions in pancreatic cancer and differently shapes stroma. (A) Tumor pancreatic fibrosis quantification in KPC/IL17A+/+ (white floating bars) and KPC/IL17A−/− mice (gray floating bars). Each symbol corresponds to a single mouse analyzed with ImageJ (n = 5 to 8 mice per group), and minimum to maximum values are represented. *P < 0.05, **P < 0.001, and ***P < 0.0001 represent significant differences in the IL17A-deficient mice compared with the IL17A-proficient mice. (B) Representative pictures of Picrosirius red staining (Upper) and of SHG microscopy analysis (Lower) of pancreatic tissues from KPC/IL17A+/+ and KPC/IL17A−/− mice at the indicated age (n = 5 to 8 mice per group). The same areas were recorded in transmission light for Picrosirius red staining and with two-photon microscopy for SHG, and collagen fibers are represented in yellow. Arrows indicate more dispersed and less compact collagen fibers, and arrowheads indicate straight and tight collagen fibers. (Scale bars, 75 μm.) (C) Representative pictures of Picrosirius red staining of pancreatic tissues from patients with high (Left) and low (Right) levels of serum IL17A. Magnification at 40× points out the differences in fibrillar structure. (Scale bars, 150 and 50 μm, respectively, at 10 and 40× magnification.) (D) A close-up view of specific genes and proteins differentially expressed in the absence of IL17A. The most relevant factors related to myeloid/granulocyte recruitment and activation (Upper Left), Th1 recruitment and activation (Lower Left), matrix remodeling (Upper Right), and cell growth (Lower Right) are grouped into four blocks. The color-coded maps represent the fold change in IL17A−/− CAFs versus IL17A+/+ CAFs and in IL17A−/− CAFs stimulated with rIL17A (10 ng/mL) versus IL17A−/− CAFs, as indicated by each closed block (n = 3 CAF bulks each). (E) Top upstream regulators predicted by IPA to be activated in the absence of IL17A. (F) Kaplan–Meier curves comparing survival of PDA patients from TCGA interrogation about genes found up-regulated (Th1 response) or down-modulated (myeloid/granulocyte recruitment) in fibroblasts from KPC/IL17A−/− mice.

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: IL17A critically shapes the transcriptional program of fibroblasts in pancreatic cancer and switches on their protumorigenic functions

doi: 10.1073/pnas.2020395118

Figure Lengend Snippet: IL17A affects fibrotic reactions in pancreatic cancer and differently shapes stroma. (A) Tumor pancreatic fibrosis quantification in KPC/IL17A+/+ (white floating bars) and KPC/IL17A−/− mice (gray floating bars). Each symbol corresponds to a single mouse analyzed with ImageJ (n = 5 to 8 mice per group), and minimum to maximum values are represented. *P < 0.05, **P < 0.001, and ***P < 0.0001 represent significant differences in the IL17A-deficient mice compared with the IL17A-proficient mice. (B) Representative pictures of Picrosirius red staining (Upper) and of SHG microscopy analysis (Lower) of pancreatic tissues from KPC/IL17A+/+ and KPC/IL17A−/− mice at the indicated age (n = 5 to 8 mice per group). The same areas were recorded in transmission light for Picrosirius red staining and with two-photon microscopy for SHG, and collagen fibers are represented in yellow. Arrows indicate more dispersed and less compact collagen fibers, and arrowheads indicate straight and tight collagen fibers. (Scale bars, 75 μm.) (C) Representative pictures of Picrosirius red staining of pancreatic tissues from patients with high (Left) and low (Right) levels of serum IL17A. Magnification at 40× points out the differences in fibrillar structure. (Scale bars, 150 and 50 μm, respectively, at 10 and 40× magnification.) (D) A close-up view of specific genes and proteins differentially expressed in the absence of IL17A. The most relevant factors related to myeloid/granulocyte recruitment and activation (Upper Left), Th1 recruitment and activation (Lower Left), matrix remodeling (Upper Right), and cell growth (Lower Right) are grouped into four blocks. The color-coded maps represent the fold change in IL17A−/− CAFs versus IL17A+/+ CAFs and in IL17A−/− CAFs stimulated with rIL17A (10 ng/mL) versus IL17A−/− CAFs, as indicated by each closed block (n = 3 CAF bulks each). (E) Top upstream regulators predicted by IPA to be activated in the absence of IL17A. (F) Kaplan–Meier curves comparing survival of PDA patients from TCGA interrogation about genes found up-regulated (Th1 response) or down-modulated (myeloid/granulocyte recruitment) in fibroblasts from KPC/IL17A−/− mice.

Article Snippet: For some experiments, PDA cells and CAFs isolated from KPC/IL17A −/− mice were stimulated in the presence or absence of different concentrations of mouse rIL17A (1, 10, and 100 ng/mL; Abnova), and supernatants and cell pellets were collected and frozen until use.

Techniques: Staining, Microscopy, Transmission Assay, Activation Assay, Blocking Assay